The dried split seeds of Momordica Charantia L. were washed , dried, powered, and deoiled thoroughly with hexane (cold extraction). The dried powder so obtained was dissolved in water and this mixture was adjusted to pH 9.5. The upper layer was decanted off and the protein was precipitated at pH. 3 with sulfuric acid. The Precipitate thus formed was collected dried, powered and washed with acetone/ ethanol again till the upper layer was clear of its turbidity. The protein (Polypeptide-K) thus collected was tested for its 100% purity and then subjected to various analytical tests.